Single-molecule study of a B-family DNA polymerase
نویسندگان
چکیده
Background: DNA polymerases use a multistep mechanism to faithfully replicate DNA. Results: Replicative DNA polymerase PolB1 binds DNA in multiple conformations which are affected by nucleotide or mismatched DNA binding. Conclusion: Modulation of conformational dynamics and DNA binding kinetics affects the replication fidelity of PolB1. Significance: Single-molecule techniques can reveal subtle differences in substrate binding properties and conformational dynamics of a DNA polymerase.
منابع مشابه
The study of dose gamma rays of 192Ir source on DNA single strand break (SSB) and DNA double strand break (DSB) in soft tissue phantom
Introduction: Passage of ionizing radiation through the organs of living creatures develops clusters of damaged nucleotides inside the DNA rounds. 192Ir Gamma source is one of the most widely used sources in brachytherapy of cervical and prostate cancer. Thus, in this research, we investigated the flux of photons and its resulting secondary electrons, the single-strand break (S...
متن کاملDetection of Human Parvovirus B19 Markers in Blood Samples of Donors
Background and Aims: Human parvovirus B19, a member of the parvoviridae family, with single-stranded DNA is a very minute non-enveloped virus. B 19 virus is mostly transmitted via the respiratory tract but some studies have been reported which B19 virus can be transmitted through blood and/or blood products. The aim of this study was to evaluate the prevalence of B19 among blood donors in Tehra...
متن کاملPolyelectrolyte surface interface for single-molecule fluorescence studies of DNA polymerase.
We report the use of polyelectrolyte multilayers in a stable robust surface chemistry for specific anchoring of DNA to glass. The nonspecific binding of fluorescently tagged nucleotides is suppressed down to the single-molecule level, and DNA polymerase is active on the anchored DNA template. This surface-chemistry platform can be used for single-molecule studies of DNA and DNA polymerase and m...
متن کاملDetection of single Dactylogyrus spp. in DNA extracted from infected gill tissue of fishes using Polymerase Chain Reaction
Dactylogyrus spp. are monogenean worms found mostly as ectoparasites on the gills of several fish species, including carp and goldfish. These parasites are commonly detected by microscopic analysis of the gill lamellae, but this is time-consuming and technically difficult. In contrast to this conventional method, molecular techniques provide specific, sensitive and safe detection of parasites. ...
متن کاملPoint Prevalence of Hepatitis B Virus Infection among Adolescents in Visakhapatnam, India
Introduction: Occurrence of detectable amounts of viral antigen or viral particles in the blood of infected patients with Hepatitis B virus (HBV) is a significant characteristic of HBV infection. Detection of HBV antigen or its DNA among individuals of a community is a crucial factor to know the burden of HBV infection. Hepatitis B surface antigen (HBsAg) is a suitable marker of HBV infection b...
متن کامل